Gene expression profile of TSLP-treated blood DCs. (A) Gene transcripts expression on purified total blood DCs directly after sorting (ex vivo) or after a 6-hour treatment with medium alone or supplemented with TSLP or TNF-α. Genome-wide expression was determined by Affymetrix chips Human Genome U133 Plus 2.0 microarray analysis. Signal intensity levels: −, ≤50; +, 50 to 500; ++, 500 to 5000; +++, ≥5000. (B) Data represent signal intensity levels for the corresponding gene transcripts under the different conditions (ex vivo: n = 5, medium; TNF-α, n = 3; TSLP, n = 4). Expression of LC-specific molecules CD1a, CD207, MMP12, and CCR6 showed a trend for TSLP-induced up-regulation and specificity but did not reach statistical significance using a Mann-Whitney nonparametric test.