TSLP + TGF-β LCs induce a Th2 profile on naive CD4⁺ T cells. BDCA-1⁺ DCs subsets were stimulated with or without TSLP and TGF-β for 24 hours. CD34⁺ cells were stimulated with Flt3-L, TNF-α, GM-CSF, and TGF-β. CD34⁺- derived LCs and BDCA-1⁺–derived LCs were sorted according to CD1a and CD207 expression and cultured with allogeneic naive CD4⁺ T cells for 6 days before T-cell restimulation. Symbols represent cells purified from the same donor. (A) T-cell expansion was assessed by calculating the ratio of the number of T cells at the end of the culture divided by the number of T cells plated at the start of the culture. **P ≤ .005, paired Student t test. Bars represent medians. (B) Data represent cytokine concentration at the end of the culture measured by multiplex bead array. *P ≤ .05; **P ≤ .005, paired Student t test. Bars represent medians. (C) PCA showing the resemblance of the naive T-cell profiles (secretion of 13 cytokines) induced under different conditions. Components 1 and 2 were selected as the axes explaining most of the data variance. The crosses represent individual donors (n = 4). The squares represent the barycenters. Confidence ellipses at 95% are depicted in each condition.