Serum and liver iron concentrations in WT and Erfe-deficient mice after injection of BA. Serum iron concentration was decreased between days 0 and 13 after injection of BA in WT and Erfe^−/− mice and was still reduced on day 21 in Erfe^−/− mice. Compared with WT mice, Erfe-deficient mice exhibited significantly lower serum iron concentrations on days 13 and 21 (A). Liver iron content (B) was increased during recovery from anemia in WT and Erfe-deficient mice except for WT mice at day 21, where iron levels are similar to day 0. Compared with WT mice, liver iron content was significantly higher in Erfe^−/− mice 21 days after infection. Data shown for serum iron concentration (A) and liver iron content (B) are means ± SEM and were compared for each time point to values for control mice at t = 0 (n = 6-7 per time point) by 2-tailed Student t test (***P < .001, **P < .01, *P < .05). Values were also compared at each time point between WT and Erfe^−/− mice by 2-tailed Student t test (^###P < .001, ^##P < .01).