Mysm1 is required for DC development. (A-H) Spleens and BM from 4- to 6-week-old WT or Mysm1^−/− mice were analyzed by FACS for the indicated cell populations (n = 4). (A) FACS analyses of splenic cDCs (CD11c^highMHCII⁺) and the subsets: CD8⁺ cDCs (CD11c^highMHCII⁺CD8⁺CD11b⁻), CD8⁻ cDCs (CD11c^highMHCII⁺CD8⁻CD11b⁺), and splenic pDCs (CD11c^intPDCA1⁺). (B) Frequency and cell numbers of splenic cDCs and pDCs from WT and Mysm1^−/− mice. (C) FACS analyses of BM pDCs (CD11c⁺PDCA1⁺). (D) Frequency and cell numbers of pDCs from BM of WT and Mysm1^−/− mice. (E) FACS analyses of splenic granulocytes (CD11b⁺Ly6g⁺Ly6c^low), monocytes (CD11b⁺ Ly6g^neg-lowLy6c^high), and macrophages (CD11b⁺Gr1⁻F4/80⁺). (F) Frequency and cell numbers of each cell type. (G) FACS analyses of BM-derived granulocytes and monocytes. (H) Frequency and cell numbers of each cell type. The data were shown form 1 of 3 independent experiments. *P < .05; **P < .01; ***P < .001.