Altered histone modification and RNA polymerase activity at Flt3 locus in Mysm1^−/− BM. ChIP assay analyzed BM Lin⁻ cells from WT or Mysm1^−/− mice via (A) anti-uH2A, (B) anti-H3K27me3, (C) anti-phosphoserine-2 RNA polymerase, or (D) anti-phosphoserine-5 RNA polymerase II. The precipitated DNA was analyzed by qPCR with primers amplifying Flt3 promoter regions (promoter 1 and 2), promoter deprived region (control primer), and Flt3 exon 1 region. *P < .05; **P < .01; ***P < .001. PDR, promoter-deprived region.