Figure 5
Figure 5. Bmi1 rescues the long-term repopulation defect caused by Ubap2l knockdown. (A) Effect of Bmi1 overexpression on the reconstitution activity of Lin−SCA1+ cells infected with shLuc or shUbap2l, 16 weeks posttransplantation. Shown is the average of 2 independent experiments with 3 different infections (n = 13 mice per condition) with the standard error of the mean. *Statistically significant difference (P = .0486). (B) Representative FACS profiles of donor cells in recipient’s BM 16 weeks posttransplantation of Lin−SCA1+ cells infected with different combinations of shUbap2l and Bmi1 cDNA. (C) Assessment of the contribution of rescued shUbap2l + Bmi1 BM cells (GFP+) to myeloid and lymphoid populations 16 weeks posttransplantation as determined by FACS using MAC1 and GR1, and B220 antibodies, respectively. The results are from 1 mouse and are representative of all rescued BM analyzed. FACS, fluorescence-activated cell sorting.

Bmi1 rescues the long-term repopulation defect caused by Ubap2l knockdown. (A) Effect of Bmi1 overexpression on the reconstitution activity of Lin−SCA1+ cells infected with shLuc or shUbap2l, 16 weeks posttransplantation. Shown is the average of 2 independent experiments with 3 different infections (n = 13 mice per condition) with the standard error of the mean. *Statistically significant difference (P = .0486). (B) Representative FACS profiles of donor cells in recipient’s BM 16 weeks posttransplantation of Lin−SCA1+ cells infected with different combinations of shUbap2l and Bmi1 cDNA. (C) Assessment of the contribution of rescued shUbap2l + Bmi1 BM cells (GFP+) to myeloid and lymphoid populations 16 weeks posttransplantation as determined by FACS using MAC1 and GR1, and B220 antibodies, respectively. The results are from 1 mouse and are representative of all rescued BM analyzed. FACS, fluorescence-activated cell sorting.

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