Deregulated hematopoiesis in DTAosb mice. (A) Whole bone marrow mononuclear cells collected by crushing 1 femur and tibia of 2-month-old WT and DTAosb mice. (B-E) Flow cytometry analysis of bone marrow cells showing representative images and percentage of LSK cells (B), myeloid cell population (CD11b+/Gr1+) (C), mature (B220+/IgM+) and immature (B220+/IgM−) B-lymphopoietic subsets (D), and erythroid progenitors (Ter119+) in the bone marrow (E). (F-G) Syngeneic 2-month-old C57BL/6 WT or DTAosb mice were injected with MLL-AF9 cells and harvested 14 days after injection. Flow cytometry analysis of bone marrow showing representative plots and percentage of LSK cells in the S-phase (F) and percentage of LT-LSK and ST-LSK cells (G). (A-E = 11-14 mice per group; F-G, n = 3 mice in WT group and n = 4 mice in DTAosb group). *P < .05 vs WT mice. LT-LSK, long-term LSK.
