Cc2^−/− platelets display greater adhesion and thrombus formation under arterial flow on immobilized type I collagen. (A-C) Rhodamine-labeled whole blood of wild-type and Cc2^−/− mice was perfused over 500 µg/mL type I fibrillar collagen–coated µ-slide III^0.1 at a shear wall flow rate of 1800 s⁻¹. Z-stack images were recorded over 4 minutes with a Zeiss Axiovert microscope captured with Axiocam MRm camera and analyzed using Zeiss Axiovision Rel4.6 software. Thrombi formed were analyzed by deconvolution and 3-D reconstructions. (A) Images of in vitro thrombus formation under arterial flow on immobilized type I fibrillar collagen for both wild-type and Cc2^−/− platelets over 4 minutes. (B) Kinetics of thrombus volume (µm³) over time was derived by multiplying thrombus area (µm²) with thrombus height (µm) for both wild-type and Cc2^−/− platelets (30 480 ± 3822 vs 12 480 ± 588.2 µm³; ***P < .001; at 4 minutes; n = 10). (C) Thrombus volume (µm³) data from (B) was stratified according to sex, both male and female, for both wild-type and Cc2^−/− platelets at the 4-minutes time point (*P < .05; n = 5 per group).