Ghr is dispensable for HSC function. (A-B) BM analysis of steady-state hematopoiesis in Ghr^fl/fl;Vav1^Cre/+ experimental and Ghr^+/+;Vav1^Cre/+ control mice showing BM frequency of (A) lineage marker negative, Sca1⁺, and c-Kit⁺ (LSK), MP, and common lymphoid progenitor (CLP) populations, and (B) LSK compartment including HSCs. (C) Experimental design for analysis of recipient mice competitively transplanted with Ghr^fl/fl;Vav1^Cre/+ and Ghr^+/+;Vav1^Cre/+ BM. (D-E) Primary transplant analysis showing (D) donor PB reconstitution and (E) donor BM reconstitution. (F-G) Secondary transplant analysis showing (D) donor PB reconstitution and (E) donor BM reconstitution. (H) Experimental overview of serial 5-FU exposure followed by competitive BM transplantation of Ghr^fl/fl;Vav1^Cre/+ experimental and Ghr^+/+;Vav1^Cre/+ control mice. (I) Analysis of white blood cell (WBC) counts after post-5-FU injection over the indicated time-course in Ghr^fl/fl;Vav1^Cre/+ (gray line) and Ghr^+/+;Vav1^Cre/+ (black line) mice. Arrows indicate time points of 5-FU injection. (J) The 1° transplant analysis showing donor reconstitution in PB at 4 and 16 weeks posttransplant. (K) Secondary transplant analysis showing donor reconstitution in PB at 4 and 16 weeks posttransplant. CBC, complete blood counts; i.p., intraperitoneal.