FOXO1 significantly activates PC signature in cHL cell lines. cHL cell lines stably expressing FOXO1(A3)ER were harvested 24 hours after treatment with 4-OHT or with vehicle. (A) Heat-map representation of the top 20 upregulated (red) and downregulated (green) genes. (B-E) Gene set enrichment analysis of genes modulated by FOXO1 in cHL cell lines. The enrichment score was calculated by a running sum statistic (green line). It increases when a gene of a gene signature (vertical black bar) is encountered by walking down the ranked list obtained by our GEP. The higher the correlation of the gene with the phenotype, the higher the increment.³⁴  (B) FOXO1 does not significantly enrich GC signature in cHL cell lines. (C) Enrichment of memory B-cell (M) signature. (D) Enrichment of signature of genes expressed fivefold higher in PCs than in HRS cells. (E) Enrichment of the PC signature. FDR, false discovery rate; NES, normalized enrichment scores.