Genes expressed in the megakaryocyte and platelet lineage correlate with EVI1 expression. (A) The candidate genes positively correlated with EVI1 expression were extracted by analyzing 2 different sets of microarray data: (i) human AML BM specimens (GSE6891) and (ii) KSL cells derived from Evi1 cKO mice (GSE11557). For human AML microarray analysis, samples were divided into EVI1-high AML (30 cases) and EVI1-low AML (430 cases) groups according to EVI1 expression as well as diagnostic information, and then 400 genes highly expressed in the EVI1-high AML group were identified (fold change > 1.4 and P < .05). From Evi1 cKO mice microarray data, 2199 genes downregulated by Evi1-knockout in KSL cells were extracted (fold change > 1.4 and P < .05). The Venn diagram revealed that 42 genes were highly correlated with EVI1 expression. (B-E) According to the gene set enrichment analysis, several gene sets related to platelet function were enriched in the EVI1-high AML group compared with the EVI1-low AML group. (B) Gene set name: REACTOME_FORMATION_OF_PLATELET_PLUG; 174 genes. (C) Gene set name: REACTOME_INTEGRIN_ALPHAIIBBETA3_SIGNALING; 23 genes. (D) Gene set name: REACTOME_PLATELET_ACTIVATION; 155 genes. (E) Gene set name: REACTOME_HEMOSTASIS; 262 genes. NES indicates the normalized enrichment score. (F) Comparison of ITGA2B expression levels between EVI1-high cases with 3q abnormalities (n = 5) and those without 3q abnormalities (n = 25) (GSE6891). The expression levels of ITGA2B were comparable irrespective of the presence of 3q abnormalities (NS, not significant; Student t test). Three probe IDs examined are described in Table 1. (G) Comparison of ITGA2B expression levels between EVI1-positive MLL-rearranged cases (n = 3) and EVI1-negative MLL-rearranged cases (n = 7) (GSE6891). ITGA2B expression levels in EVI1-positive MLL-rearranged cases were higher than those in EVI1-negative MLL-rearranged cases (*P < .05, Student t test).