Expression of JAK3 M511I in the bone marrow cells of Balb/c mice cause a T-lymphoproliferative disease that progresses to T-ALL. (A) Evolution of the WBC count in JAK3 M511I animals. WBC count was measured every 2 weeks. The upper limit of normal WBC count (10 000/mm3) is indicated by a dashed line. (B) Kaplan-Meier survival curve of BALB/c mice receiving bone marrow transplantation of lineage-negative cells expressing JAK3 M511I. Mice transplanted with cells expressing wild-type JAK3 had neither hematologic abnormalities nor signs of disease. (C) The spleen and thymus weights of mice transplanted with cells expressing JAK3 M511I compared with mice transplanted with JAK3 wild-type cells. Significance was determined by Student t test. (D) Hematoxylin and eosin (H&E) staining of the spleen, bone marrow, thymus, and lymph node. Terminal deoxynucleotidyl transferase (TdT) staining of the thymus and lymph node. The scale bar represents 100 μm. (E) Analysis of the bone marrow cells of diseased animals by flow cytometry with anti-CD4 and anti-CD8 antibodies. Pathology images were obtained using a Leica DM2500 microscope and a Leica DFC290HD camera and analyzed using the Leica Application suite LAS v4.1 software. Images were processed with Adobe Photoshop CS5.
