Figure 3
![Figure 3. Specificity of all 29 anti-ADAMTS13 mAbs toward recombinant ADAMTS13 and control antigens at equimolar concentrations assessed by ELISA. Black bars represent absorbance at 450 nm as an average of the signal obtained for each mAb separately within the corresponding CDR3 motifs M-1 to M-8. Antigens (human rADAMTS13, human factors VII [hVII], VIII [hVIII], and IX [hIX]) and tetanus toxoid (TT) were coated at equimolar concentration on an ELISA plate. Anti-ADAMTS13 mAb I-9 (gift of Jan Voorberg, Amsterdam, The Netherlands) served as a positive control and the secondary antibody (II-Ab) as a negative control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/124/23/10.1182_blood-2014-04-561142/4/3469f3.jpeg?Expires=1735811958&Signature=dSw2D~EQVEHS8LNleqC~SmlKC35RoXyN3WyHhoByOVo2k1ruYXjnJZacomMQ5bRt4ZNOBAUWV~n6eqjGEb4jjpz~7oMzEBJ9dPWRiTeVypRJ~-hfZp6ZeO7KQ1RkMYtkj-6cnjuXrXd~NZ~XbAKPMbsOK-vRrlgzO-a9bcxxGCClMI6Spgvw007fHQGWM5sZKitILBUQUCAMjGaussd4Otal-Ng2Pi1HbEz7z4DyWaJz1M3l04s6j3lwRydYwjDGnwhGBKwKPh-rQCZHeTevzdu~AnvfA~rSu76qpXs7isoyrhNCZjr5eVcwXFpa8pUc9LUHcvlzNnvH-1FxqtxqSg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of all 29 anti-ADAMTS13 mAbs toward recombinant ADAMTS13 and control antigens at equimolar concentrations assessed by ELISA. Black bars represent absorbance at 450 nm as an average of the signal obtained for each mAb separately within the corresponding CDR3 motifs M-1 to M-8. Antigens (human rADAMTS13, human factors VII [hVII], VIII [hVIII], and IX [hIX]) and tetanus toxoid (TT) were coated at equimolar concentration on an ELISA plate. Anti-ADAMTS13 mAb I-9 (gift of Jan Voorberg, Amsterdam, The Netherlands) served as a positive control and the secondary antibody (II-Ab) as a negative control.
