Reduced metastasis in Nbeal2^−/− mice. Lung metastasis was induced by tail vein injection of 5 × 10⁵ mouse melanoma B16-F10 cells. Lungs were taken after 10 days and the number of pulmonary metastases were counted on all 5 lobes of the lung. Representative lung metastases in control (n = 9) and Nbeal2^−/− mice (n = 5) (A) and quantitation (B). Bar represents mean ± standard error of the mean. *P = .0001.