DNT share CDR3 sequences with CD4⁺ and CD8⁺ TEMRA cells. (A) DNT and different SPT-cell populations of 1 ALPS-FAS patient were sorted for TCRβ deep sequencing. Equal amounts of genomic DNA were amplified by multiplex PCR. The number of resulting reads and unique clonotypes are shown for each cell population. (B) CDR3 lengths distribution is shown for all clonotypes within each T-cell population. (C) Percentage of overlapping identical sequences among the different T-cell populations. (D) CDR3 lengths distribution was analyzed among overlapping sequences between CD4⁺CD57⁺ and DNT, as well as between CD8⁺CD57⁺ and DNT.