Double mutated T cells accumulate among CD4 and CD8 TEMRA cells in patients with ALPS-FAS-sLOH. (A) Fas expression is shown on naïve (naïve, CCR7⁺CD45RA⁺), central memory (CM, CCR7⁺CD45RA⁻), effector memory (EM, CCR7⁻CD45RA⁻), and terminally differentiated CD8⁺ T cells (TEMRA, CCR7⁻CD45RA⁺), and (B) on CD3⁺TCRαβ⁺CD4⁻CD8⁻ DNT of 2 patients with ALPS-FAS-sLOH (P1: 14 y, P2: 32 y) compared with an adult control. (C) CD57 and Fas expression on CD4⁺, CD8⁺, and DNT of the same patients compared with an adult control.