Zebrafish miR-142-3p is enriched in myeloid cells and the definitive hematopoietic organ. (A) The stem-loop structure of pre-miR-142a and pre-miR-142b in zebrafish. The mature miR-142-3p sequence is boxed with a red dashed line. Mature miR-142a-5p and miR-142b-5p are boxed with a black dashed line. Different nucleotides are marked in red. (B) Multiple sequence alignment of miR-142-3p (top) and miR-142-5p (bottom) from different species. Fully conserved nucleotides are indicated with an asterisk. (C) Synteny of the miR-142 loci between the zebrafish and human. (D) Phylogenic analysis of miR-142. A phylogenic tree was constructed from a clustalW2 alignment using the neighbor-joining algorithm. (E-F) WISH with DIG-labeled LNA for miR-142-3p in zebrafish embryos at 3 dpf or 5 dpf. (A) miR-142-3p is specifically expressed in CHT (red triangle) and the thymus (black triangle) at 3 dpf. (B) miR-142-3p is expressed in CHT (red triangle), the kidney (yellow triangle), the thymus (black triangle), and the olfactory bulbs (white triangle) at 5 dpf. Expression of miR-142-3p in the tissue mesenchymes is indicated with a green triangle. (G-H) WISH for miR-142a-5p in zebrafish embryos at 3 dpf or 5 dpf. (I-J) qPCR analysis for mi-142-3p and miR-142a/b-5p in GFP-positive cells from Tg(cmyb:eGFP) (I) or Tg(zlyz:eGFP) (J) zebrafish at 3 dpf. **P < .01 vs GFP-negative cells; 2-tailed Student t test.