Net ex vivo expansion of cultured human umbilical cord blood CD133⁺ cells as determined by limiting dilution analysis. (A-B) Numbers of total nucleated cells (A) and CD34⁺ cells (B) before and after culture with 25 µg/mL coated anti-LILRB2 pAb. (C-D) Percentages of donor human CD45⁺ cells (C) in the peripheral blood at 1 and 2 months and (D) in bone marrow in recipient NSG mice transplanted with uncultured or expanded cells. (E) Net expansion of HSCs as determined by limiting dilution analysis. The numbers of input equivalent cells were used in the calculation. (F-I) Comparisons of multilineage repopulation of HSCs before and after ex vivo expansion. *P < .05; **P < .01; n = 8. MNC, mononuclear cell; n.s., not significant.