Integrin α₃β₁ regulates TLR2-, but not TLR4-induced cytokine responses. (A) ELISA of IL-6, TNF-α, and IL-10 in supernatants of neutrophils, isolated by negative selection, from BM of α₃ cKO (Itga3^flox/flox; Ela-Cre) mice (white bars) and control Ela-Cre (Itga3^wt/wt; Ela-Cre) mice (black bars). Cells (10⁶) were stimulated for 24 hours with Pam₃CSK₄ (100 ng/mL) and supernatants tested in duplicate. The results from 3 independent experiments are expressed as mean ± SD. *P < .05 (Mann-Whitney test). (B) ELISA of IL-6, TNF-α, and IL-10 in supernatants of neutrophils, isolated by negative selection, from BM of α₃ cKO (white bars) and control (black bars) mice. Cells (10⁶ cells/300 μL) were stimulated for 24 hours with ultrapure LPS (100 ng/mL) and supernatants tested in duplicate. The results from 3 independent experiments are expressed as mean ± SD. *P < .05 (Mann-Whitney test).