Figure 2
Figure 2. IPI-145 abrogates the prosurvival AKT signal in XLA-BTK C481S cells. The inducible XLA-BTK wild-type (WT) and XLA-BTK C481S cell lines were induced with 1 μg/mL of doxycycline for 48 hours. Both cells were left untreated or treated with IPI-145 at various concentrations (0.01 to 1 μM) for 1 hour or with ibrutinib (0.5 μM and 1 μM) for 30 minutes followed by washout and incubated in media for another 30 minutes. Cell lysates were immunoblotted for pBTKY223, total BTK, pATKS473, total AKT, and GAPDH. The blots are representative of 3 independent experiments.

IPI-145 abrogates the prosurvival AKT signal in XLA-BTK C481S cells. The inducible XLA-BTK wild-type (WT) and XLA-BTK C481S cell lines were induced with 1 μg/mL of doxycycline for 48 hours. Both cells were left untreated or treated with IPI-145 at various concentrations (0.01 to 1 μM) for 1 hour or with ibrutinib (0.5 μM and 1 μM) for 30 minutes followed by washout and incubated in media for another 30 minutes. Cell lysates were immunoblotted for pBTKY223, total BTK, pATKS473, total AKT, and GAPDH. The blots are representative of 3 independent experiments.

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