Fanca deficiency affects pro-platelet formation. (A) Pro-platelet production in vitro and representative images presenting CD41⁺Lin⁻ WT and Fanca^−/− cells at day 4 of culture (n = 4 mice per group, 250 alive MKs per point were scored, *P < .05, Student t test; scale bar = 50 μm). (B) Representative flow cytometry plots showing the gating strategy to identify different progenitors. (C) Number of Lin⁻Sca-1⁺c-Kit⁺ (LSK) cells and myeloid progenitors (CMPs, GMPs, and MEPs) in the BM of Fanca^−/− and WT mice (n = 5 per group, results presented as means ± SEM; LSK, P = .2605; CMP, P = .4895; GMP, P = .6598; MEP, P = .8014).