HOXA9-MEIS1–induced AML shows no difference in LIC self-renewal or phenotype depending on HIF-1α. (A) Kaplan-Meier survival curves of mice transplanted with HoxA9-Meis1–expressing BM cells derived from primary recipients. Cells derived from 2 different donors were used to transplant 4 × 105 cells into secondary recipients (n = 12). The log-rank (Mantel-Cox) test was used to assess statistical significance. Leukemic animals derived from 2 independent experiments were sacrificed at week 3 after transplantation and WBC count (n = 20) (B); percentage of myeloid cells in PB (C), BM, and spleen (D); and spleen and liver weights (E) analyzed for evaluating the stage of the disease (n = 11). Plots represent mean ± SEM. Two-tailed Student t test was used to assess statistical significance. **P < .01, ***P < .001.
