HIF-1α status does not generally influence cell cycle or metabolism of leukemic cells, but its deletion increases apoptosis. (A) Cell-cycle analysis of the leukemic cells (GFP⁺ or myeloid, depending on the model) from the different indicated organs of diseased mice from 2 independent experiments (AE9a: n = 7; HoxA9-Meis1: n = 11; MLL-AF9: n = 11). (B) Apoptosis analysis of the leukemic cells (GFP⁺ or myeloid, depending on the model) from the different indicated organs of diseased mice (HoxA9-Meis1: n = 8; MLL-AF9: n = 7). (C) Analysis of mitochondrial activity, mitochondrial ROS, and total ROS. Median fluorescence intensity (MFI) of MitoTracker-stained (n = 7-11), MitoSOX-stained (n = 7-8), or CellROX-stained (n = 7-11) leukemic cells (GFP⁺ cells in AE9a and MLL-AF9 models and myeloid cells in HOXA9-MEIS1 model) from diseased secondary recipients in BM and spleen was normalized to the mean control values. Plots represent mean ± SEM. Two-tailed Student t test was used to assess statistical significance. *P < .05, **P < .01.