Isolation of BFU-E and CFU-E cells by cell-sorting using CD34, CD36, and IL-3R as markers. (A) Colony forming ability of the sorted IL-3R⁺ and IL3R⁻ cells. (B) Colony forming ability of the sorted IL-3R⁻CD34⁺CD36⁻ in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (C) Colony forming ability of the sorted IL-3R⁻CD34⁻CD36⁺ cells in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (D) Expression of surface markers of sorted IL-3R⁻CD34⁺CD36⁻ and IL-3R⁻CD34⁻CD36⁺ cells. The surface expression of indicated proteins was measured by flow cytometry. The representative profiles are shown. Gray line: autofluorescence control from unstained cells; black line: fluorescence from cells stained with indicated antibody. Results from 3 independent experiments are shown.