Overexpression of TF mRNA and protein in PARP-14–deficient macrophages with stabilization of TF mRNA. WT and Parp14^−/− BMDM were treated with LPS (1 µg/mL) and then analyzed for TF expression at the times shown by (A) RT-PCR, with mRNA levels normalized to unstimulated WT cells (n = 5 experiments). (B) Western blot analysis, with WT and Parp14^−/− lysates were run on the same gels and processed simultaneously, but separated for clarity. The figure shows representative blots of five independent experiments. (C) TF mRNA decay in WT and Parp14^−/− macrophages after addition of actinomycin D (5 µg/mL) to cultures that had been treated with LPS for 2 hours (n = 6 experiments). Data in (A) and (C) are expressed as mean ± SEM. *P < .05; ***P < .001 analyzed using a 2-tailed Student t test. ActD, actinomycin D; Rel, relative.