Plasma membrane and Golgi apparatus colocalization images for mutated hVKORC1 di-arginine and di-lysine variants in HEK293T cells. This figure shows colocalization images for single HEK293T cells of immunostained plasma membrane and Golgi apparatus for all hVKORC1 variants investigated in this study. The left column shows merged images in which the nucleus is stained by ToPro3 (blue), the respective EGFP-tagged hVKORC1 variant (green), and the plasma membrane stained by CellMask orange in red. The merged images are followed by images demonstrating the hVKORC1 variant and stained plasma membrane only. On the right side, there are also merged images of the nucleus and the hVKORC1 variant demonstrated in the same way and colors as for the left side. The red fluorescent signal shows the stained Golgi apparatus (anti Golgin-97, AlexaFluor594). The merged images are followed again by images showing the hVKORC1 variant and the stained Golgi apparatus only. No colocalization with the plasma membrane as well as with the Golgi apparatus was found for all hVKORC1 variants investigated including the wt.