Cryoelectron tomography depicting actin network. (A) CS2 parental line: Section through cryoelectron tomogram showing a part of an RBC infected with P falciparum (left) in a trophozoite stage, and corresponding surface rendered view (right). The insets in the middle show views extracted at different Z-planes and orientations of the tomogram, and are indicated by boxes and marked accordingly (1-4). Actin filaments are indicated with arrows. Scale bar: 100 nm. (B) CS2ΔPTP1 cell line: Section through cryo-electron tomogram showing a part of an RBC infected with P falciparum CS2ΔPTP1 (left) in a trophozoite stage, and corresponding surface rendered view (right). The inserts in the middle show views extracted at different Z-planes and orientations of the tomogram, and are indicated by boxes and marked accordingly (1-5). Actin filaments are indicated with arrows. Color code in both figures: RBC membrane (dark blue); knobs (red); vesicles (cyan); MC (cyan); filaments (yellow); indicated by arrows. *Edge of a hole in EM-grid carbon support. Scale bar: 100 nm. (C) Transmission electron micrograph image of negatively stained human actin filaments in the absence (top image) or presence (bottom image) of PTP1 C-terminal peptide. Scale bar represents 100 nm. (D) A significant difference of F-actin length polymerized in vitro is observed in the absence (green line) or presence of scrambled PfPTP1 peptide (red) vs presence of the peptide PfPTP1 (100 μM) (blue line).