Interaction partners of PfPTP1. (A) Affinity purification of interacting partners on CS2/PTP1-HA cell line with α-HA coupled beads. Eluted fractions were subjected to western blot analysis and blot was probed with α-HA antibody (top panel); α-SBP1 antibody (second panel); α-PfEMP1 antibody (third panel) and α-PIESA2 (bottom panel). (B) Reciprocal immunoprecipitation of interacting partners of PfPTP1 in CS2 parental line with α-SBP1 antibody or rabbit normal serum (NRS; negative control). The western blot was probed with α-PfPTP1 antibodies. The arrow points to the specific band for PfPTP1. The 25 kDa band represents the light chain of the SBP1 antibody. (C) Blue native PAGE on CS2/PTP1-HA cell line. The first dimension was run under native conditions, the strip then excised and run in the second dimension under denaturing conditions to resolve potential complexes. The blot was first probed with α-SBP1 antibody and HRP-conjugated secondary antibody, then stripped and probed with α-HA antibody and specific complexes detected via electrochemiluminescent reagents and x-ray film.