Morphologic and cytogenetic analysis of BM sample of the APL case with t(3;17)(q26;q21),t(7;17)(q11.2;q21) translocation. (A) The May-Giemsa staining. BM cells were shown to be blocked at the hypergranular promyelocytic stage lacking Auer rods. (B) Myeloperoxidase staining. (C) Specific esterase staining. (D) Nonspecific esterase staining. (B-D) Cellular histochemical staining examination exhibited high positive rates of blast cells. (E) FISH analysis. PML probe (orange signal) and RARα probe (green signal) were used. No PML-RARα was detected. (F) Karyotype analysis. 46,XY,t(3;17)(q26;q21),t(7;17)(q11.2;q21) was found in 14 metaphases among 15 metaphases detected. (G) Karyotype analysis of relapsed BM cells. A more complex recurring chromosomal transcription, 46,XY,t(3;17)(q26;q21),add(5)(q32),del(6)(q22q24),t(7;17)(q11.2;q21),add(10)(q26),del(11)(p14)[13]/46,XY[1], was observed in 13 metaphases among 14 metaphases detected. The rearrangements and break points in panels F and G were indicated by arrows.