LPS-induced emergency granulopoiesis is abrogated in Tie2-Cre;Myd88^fl/fl mice. (A) Frequency and absolute number of PB CD11b⁺ cells after LPS stimulation in control Myd88^fl/fl, and experimental Nes-Cre;Myd88^fl/fl, Pdgfrb-Cre;Myd88^fl/fl, Alb-Cre;Myd88^fl/fl, and Tie2-Cre;Myd88^fl/fl mice. (B) BM cellularity after LPS stimulation in control Myd88^fl/fl, and experimental Nes-Cre;Myd88^fl/fl, Pdgfrb-Cre;Myd88^fl/fl, Alb-Cre;Myd88^fl/fl, and Tie2-Cre;Myd88^fl/fl mice. (C) Representative FACS profile showing characteristic LPS-induced changes in BM CD11b⁺Gr1^high mature and BM CD11b⁺Gr1^low immature neutrophils in control Myd88^fl/fl, and experimental Nes-Cre;Myd88^fl/fl, Pdgfrb-Cre;Myd88^fl/fl, Alb-Cre;Myd88^fl/fl, 0and Tie2-Cre;Myd88^fl/fl mice. (D) Frequencies and absolute numbers of BM CD11b⁺Gr1^high mature and (E) BM CD11b⁺Gr1^low immature neutrophils, and (F) plasma G-CSF levels in the different sets of tissue-specific Myd88^−/− mice after systemic LPS injection. Black squares, PBS-injected mice; red squares, LPS-injected mice; rel., relative; abs., absolute. Data from at least 3 independent experiments are shown. Two-tailed Student t tests were used to assess statistical significance (*P < .05, **P < .01, ***P < .001).