Endothelial cell–intrinsic MYD88 signaling is required to efficiently stimulate emergency granulopoiesis during systemic E coli infection. (A) Frequency and absolute number of PB CD11b⁺ cells, (B) BM cellularity, (C) frequencies and absolute numbers of BM CD11b⁺Gr1^high mature and (D) BM CD11b⁺Gr1^low immature neutrophils, and (E) plasma G-CSF levels in Myd88^fl/fl mice and Tie2-Cre;Myd88^fl/fl mice in steady-state (black squares) and after infection with 4.5 × 10⁸E coli CFU (red squares) given IP. Data from 2 independent experiments are shown. rel., relative; abs., absolute. Two-tailed Student t test was used to assess statistical significance (**P < .001, ***P < .001).