SLAP/SLAP2 limit pathological thrombus formation. (A) Unaltered hemostasis in Slap^−/−/Slap2^−/− mice. Tail bleeding times of WT and Slap^−/−/Slap2^−/− mice in saline at 37°C. Each symbol represents 1 animal. (B) The abdominal aorta was mechanically injured using a forceps (compression for 10 seconds) and blood flow was monitored by an ultrasonic flow probe until complete vessel occlusion for at least 5 minutes, or for a maximum of 30 minutes. Each symbol represents 1 animal. (C) The right carotid artery of WT and Slap^−/−/Slap2^−/− mice was injured by topical application of 2.5% FeCl₃ for 90 seconds and blood flow was monitored with a Doppler flow probe until complete vessel occlusion for at least 2 minutes, or for a maximum of 30 minutes. Each symbol represents 1 animal. (D) WT mice were treated with a platelet-depleting anti-GPIbα antibody. Platelet-depleted WT mice were transfused with WT or Slap^−/−/Slap2^−/− (DKO) platelets and 7.5% FeCl₃ were topically applied to the right carotid arteries of these mice for 1 minute. Time to complete vessel occlusion was determined as described for (C). Each symbol represents 1 animal. **P < .01. DKO, double knockout; NS, not significant.