SLAP/SLAP2 deficiency dramatically aggravates neurologic damage after focal cerebral ischemia. Infarct volumes (A) and functional outcome (B-C) 24 hours after focal cerebral ischemia in WT and Slap^−/−/Slap2^−/− mice were investigated in a murine model of ischemic stroke. Mice were subjected to 30 minutes of tMCAO. (A) Brain infarct volumes in WT (n = 9) and Slap^−/−/Slap2^−/− (n = 12) mice were measured by planimetry (left). Results represent mean ± SD. Representative images of 3 coronal brain sections stained with 2,3,5-triphenyltetrazolium chloride for 24 hours after 30 minutes of tMCAO (right). Arrows indicate infarcted areas in Slap^−/−/Slap2^−/− mice. Bederson score (B) and grip test (C) were determined 24 hours after tMCAO. Each symbol represents 1 animal. (D) SLAP/SLAP2 deficiency dramatically increases microvascular thrombosis after 30 minutes of tMCAO. Representative hematoxylin and eosin stains from the ipsilesional hemispheres of WT (n = 6) and Slap^−/−/Slap2^−/− (n = 8) mice (left), and determination of the percentage of occluded vessels (right). Number of thrombotic vessels was significantly increased in Slap^−/−/Slap2^−/− mice (arrows), whereas the microvascular patency was largely preserved in WT mice (arrowheads). Images were obtained at ×40. Bar represents 50 μm. *P < .05; **P < .01; ***P < .001.