LAMP1 and LAMP2 do not localize predominantly to dense granules in platelets. Platelets from WT mice were fixed, permeabilized, and labeled with a rabbit antibody to the LAMP1 cytoplasmic domain together with rat monoclonal antibodies to LAMP1 (A), LAMP2 (B), or multidrug resistance protein 4 (MRP4) (C and F), or with a rabbit antibody to syntaxin 13 (STX13) and rat monoclonal antibodies to LAMP1 (D) or MRP4 (E) and fluorophore-conjugated secondary antibodies. Platelets were then analyzed by deconvolution immunofluorescence microscopy. Shown are 4 single-plane images of 1 or 2 platelets each labeled by each antibody combination. (F) Shown are 5 sequential z planes (separated by 0.2 μm) and a 3-dimensional (3D)-rendered model of a single platelet labeled for LAMP1 and MRP4. Bar represents 1 μm.