Effects of the NL and RUX combination on BCR-ABL kinase activity. CML CD34⁺ cells were either left UT, or treated for 8 hours with NL (5 µM) or RUX (1000 nM) or their combination, prior to protein extraction and measurement of (A) p-BCR-ABL (p-c-Abl^Tyr245) (n = 2), and (B) p-CrkL^Tyr207 (n = 4, 2 representative blots shown) levels by western blot. SH-PTP2 (A) and β-tubulin (B) were used as loading controls.