Analyzing the effect of PRC2 accessory factor depletion in competitive transplantation assays. (A) Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of PRC2 accessory factor expression in HSPCs (Lin^–Sca1⁺c-kit⁺) isolated from E14.5 FLs, ESCs, and E14.5 murine embryonic fibroblasts (MEFs) from C57BL/6 mice. Results are normalized to housekeeping genes Hprt and Hmbs, expressed relative to an HSPC sample. Data are shown as mean ± standard deviation (SD); n = 3. (B) Design of FL competitive transplantation assays. (C) The relative contribution of shRNA-transduced cells to the B, T, and myeloid cell lineages in the peripheral blood at 10 weeks posttransplant for validated hairpins targeting PRC2 accessory factors compared with a NS control. Relative contribution is defined as the output:input ratio for each test hairpin normalized to the output:input ratio for NS controls from the same cohort. (D) Relative contribution of FL cells transduced with shRNAs against Jarid2-interacting H3K9 methyltransferases at 10 weeks posttransplant. Data from recipients transduced with hairpins targeting the same gene have been combined. Graphs shows mean + SD; recipient mouse numbers per hairpin are shown. **P < .01, ***P < .001, and ****P < .0001 were calculated by using a two-way analysis of variance (ANOVA) with multiple comparisons in GraphPad Prism5 on logit-transformed data (supplemental Data).