Adult HSPC function is sensitive to Jarid2 depletion. (A) RT-qPCR analysis of Jarid2 expression in LT-HSCs, ST-HSCs, and MPPs purified from E14.5 FL or 8-week-old adult BM from C57BL/6 mice. Results are normalized to housekeeping gene Hprt, expressed relative to a calibrator FL LT-HSC sample. Data represent mean ± SD; n = 3. (B) Experimental design for adult HSPC retroviral infection and transplantation into lethally irradiated recipients. (C) Peripheral blood analysis showing the relative contribution of transduced cells to the T, B, and myeloid cell lineages (as in Figure 2), at 6 and 10 weeks posttransplant. (D) Relative contribution (mean + SD) of transduced adult BM HSPCs to major cell types within the BM, spleen, and thymus (as in Figure 2), at 16 weeks posttransplant. Recipients are from 2 independent cohorts. *P < .05, **P < .01, and ***P < .001 were calculated by using a two-way ANOVA with multiple comparisons in GraphPad Prism5 on logit-transformed data.