American Society of Hematology

Figure 5

$Figure 5. Comparison of mTF-dependent binding and in vitro activity of mFVIIa and mFVIIa chimeras. (A) Using a PT clotting assay, mFVIIa and mFVIIa-FMR exhibit similar activity. Asterisk indicates P < .05 vs mFVIIa or mFVIIa-FMR. Data are expressed as mean ± SD. NS, nonstatistically significant difference. (B) Binding of mFVIIa (●) or mFVIIa-FMR (☐) on CHO-K1 cells expressing mTF. Increasing concentration of ligand (10-250 nM) was incubated on CHO-K1 or CHO-K1-mTF cells, and the bound fraction was analyzed as described in Figure 3. Both ligands exhibit similar mTF binding. Depicted curves for each protein are fitted from 3 independent experiments. Data points are shown as mean ± SD. (C) Generation of mFXa over time on CHO-K1 cells expressing mTF following incubation of mFVIIa (●) or mFVIIa-FMR (☐). Both ligands exhibit similar rates of mFXa generation. Depicted curves for each protein are fitted from 3 independent experiments. Data points are shown as mean ± SD. (D) Mouse thrombin generation assay following the addition of 25 nM mFVIIa (●) or mFVIIa-FMR (☐) or buffer (△) in mouse hemophilia B plasma. *P < .05 vs mFVIIa of the entire fitted data. (Inset) Endogenous thrombin potential (ETP) following addition of different concentration of mFVIIa (black bar) or mFVIIa-FMR (white bar).$

Comparison of mTF-dependent binding and in vitro activity of mFVIIa and mFVIIa chimeras. (A) Using a PT clotting assay, mFVIIa and mFVIIa-FMR exhibit similar activity. Asterisk indicates P < .05 vs mFVIIa or mFVIIa-FMR. Data are expressed as mean ± SD. NS, nonstatistically significant difference. (B) Binding of mFVIIa (●) or mFVIIa-FMR (☐) on CHO-K1 cells expressing mTF. Increasing concentration of ligand (10-250 nM) was incubated on CHO-K1 or CHO-K1-mTF cells, and the bound fraction was analyzed as described in Figure 3. Both ligands exhibit similar mTF binding. Depicted curves for each protein are fitted from 3 independent experiments. Data points are shown as mean ± SD. (C) Generation of mFXa over time on CHO-K1 cells expressing mTF following incubation of mFVIIa (●) or mFVIIa-FMR (☐). Both ligands exhibit similar rates of mFXa generation. Depicted curves for each protein are fitted from 3 independent experiments. Data points are shown as mean ± SD. (D) Mouse thrombin generation assay following the addition of 25 nM mFVIIa (●) or mFVIIa-FMR (☐) or buffer (△) in mouse hemophilia B plasma. *P < .05 vs mFVIIa of the entire fitted data. (Inset) Endogenous thrombin potential (ETP) following addition of different concentration of mFVIIa (black bar) or mFVIIa-FMR (white bar).

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