American Society of Hematology

Figure 2

$Figure 2. PAR agonist induces translocation and phosphorylation of Akt in platelets. Washed human platelets were stimulated with 2MeSADP (100 nM) (A), AYPGKF (500 μM) (B), or SFLLRN (5 µM) (C) for different time periods. Akt translocation and phosphorylation (Ser473 and Thr308) were evaluated by subjecting platelet membrane fractions to SDS-PAGE. β3-integrin was used as the lane-loading control. (D) Membrane fractions from platelets stimulated with AYPGKF were probed with anti-Akt isoform-specific antibodies. The western blot analysis shown is representative of 3 independent experiments using anti-Akt mouse mAb (Santa Cruz Biotechnologies).$

PAR agonist induces translocation and phosphorylation of Akt in platelets. Washed human platelets were stimulated with 2MeSADP (100 nM) (A), AYPGKF (500 μM) (B), or SFLLRN (5 µM) (C) for different time periods. Akt translocation and phosphorylation (Ser473 and Thr308) were evaluated by subjecting platelet membrane fractions to SDS-PAGE. β₃-integrin was used as the lane-loading control. (D) Membrane fractions from platelets stimulated with AYPGKF were probed with anti-Akt isoform-specific antibodies. The western blot analysis shown is representative of 3 independent experiments using anti-Akt mouse mAb (Santa Cruz Biotechnologies).

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