American Society of Hematology

Figure 5

$Figure 5. Role of Gq signaling pathways in PAK translocation in response to AYPGKF in human platelets. (A) Washed human platelets were stimulated with AYPGKF (500 μM) for different time periods. Translocation and phosphorylation of PAK were evaluated by subjecting platelet membrane fractions to SDS-PAGE analysis. β3-integrin was used as the lane loading control. (B) Washed human platelets were stimulated with AYPGKF (500 μM) in the presence or absence of 50 nM YM254890, and the effect on PAK translocation and phosphorylation was evaluated using western blot analysis of platelet membrane fractions. β3-integrin was used as the lane loading control. The western blot analysis shown are representative of 3 independent experiments.$

Role of G_q signaling pathways in PAK translocation in response to AYPGKF in human platelets. (A) Washed human platelets were stimulated with AYPGKF (500 μM) for different time periods. Translocation and phosphorylation of PAK were evaluated by subjecting platelet membrane fractions to SDS-PAGE analysis. β₃-integrin was used as the lane loading control. (B) Washed human platelets were stimulated with AYPGKF (500 μM) in the presence or absence of 50 nM YM254890, and the effect on PAK translocation and phosphorylation was evaluated using western blot analysis of platelet membrane fractions. β₃-integrin was used as the lane loading control. The western blot analysis shown are representative of 3 independent experiments.

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