3D telomere dynamics of multinucleated RS-like cells in the Burkitt lymphoma cell line BJAB-tTA-LMP1. Telomere distribution according to size. Results are based on 3D analysis of 30 cells for each time point. Frequency (y-axis) and relative fluorescent intensity (ie, size of telomeres) (x-axis) reveal individual telomere profiles for each time point. Telomeres with a relative fluorescent intensity (x-axis) ranging from 0 to 5000 units are classified as very short, intensity from 5000 to 15 000 units as short, intensity from 15 000 to 30 000 units as mid-sized, and intensity >30 000 units as large. LMP1 expression induces multinucleated RS-like cells with abundant very short and short telomeres when compared with mononuclear cells at day 21.