3D identification of disturbed nuclear telomere organization in a trinuclear LMP1-expressing RS-like BJAB-tTA-LMP1 cell. (A) 3D reconstruction of nuclear DNA (DAPI; blue) in surface mode reveals a small folded satellite nucleus (arrow), a second nucleus at the upper right and a third large nucleus at the bottom. (B) 3D telomere (red) reconstruction in surface mode reveals abundant telomeres in lower part of the small satellite nucleus (arrow) and irregular telomere distribution in the large nucleus. (C) Combined 3D nuclear staining confirms telomere rich DNA dense satellite nucleus (arrow) and uneven telomere distribution in the large nucleus. (D) Inset (folded satellite nucleus) localizes short telomeres and also aggregates to its lower part. Further 3D telomere analysis of this satellite nucleus is shown in supplemental Figure 2B.