Experimental design and reproducibility. (A) Flow diagram showing the experimental designs of the lentiviral shRNA screen and validation experiments. (B) K562^S and K562^R cells were infected with the Cellecta HM1 library and cultured in puromycin (see “Materials and methods”). Independent experiments were performed at the Huntsman Cancer Institute (R1, R2, R3, and HCI) or Cellecta. Comparison of the fold-change of barcodes between experiments using either K562^S or K562^R cells reveals a high level of correlation.