Enhanced RAN/XPO1 shuttling activity is associated with enhanced levels of cytoplasmic SET in TKI-resistant CML cell lines. (A) Whole cell, nuclear, and cytoplasmic lysates of K562^S, K562^R, AR230^S, and AR230^R cells (n = 3) either untreated or treated with 1 μM imatinib were separated by SDS-PAGE and analyzed for SET expression and subcellular localization by immunoblot analyses. The α-tubulin and lamin B fractionation blots overlap with that of Figure 4A. (B) Whole cell, nuclear, and cytoplasmic lysates of K562^S, K562^R, AR230^S, and AR230^R cells (n = 3) expressing shRAN in the presence or absence of doxycycline were separated by SDS-PAGE and analyzed for SET expression and subcellular localization.