Immortalized β3ΔRGT ECs exhibit reduced migration and angiogenic sprouting. (A-B) Cell migration across vitronectin-coated Transwells. (C-D) Representative images of fibrin gel bead assays to assess EC sprouting from day 1 to day 3; bead diameter, 60-87 μm. (E-F) Quantification of EC sprouting in the 3-dimensional fibrin gel bead assay. Data represent means ± SEM of 4 experiments. *P < .05; NS, not significant (paired Student t test); OD, optical density.