American Society of Hematology

Figure 2

$Figure 2. Increase in the most quiescent HSC population in Cdk6−/− mice. (A) LSK BM cells are subdivided into 3 populations based on CD150 and CD48 expression, LSKCD150+CD48− (A), LSKCD150+CD48+ (B), and LSKCD150−CD48+ (C). The LSKCD150+CD48− (A) population can be further subdivided into CD135−CD34− (HSC) and CD135−CD34+ (MPP1) subsets. Sets of representative FACS blots of Cdk6+/+ and Cdk6−/− BM cells are shown. (B) Total cell numbers of LSKCD150+CD48− (A), LSKCD150+CD48+ (B), CD150−CD48+ (C) in Cdk6+/+ and Cdk6−/− animals are shown (n = 3 per genotype). (C) Analysis of individual subpopulations of LSKCD150+CD48− (A) cells is depicted. Population A is further subdivided into HSC and MPP1 (n = 3 per genotype; *P < .05). (D) Cell-cycle distributions of fraction A cells were analyzed with DAPI and Ki-67 staining. One representative FACS blot per genotype is depicted (left). Summary of data (right; n = 6 per genotype).$

Increase in the most quiescent HSC population in Cdk6^−/− mice. (A) LSK BM cells are subdivided into 3 populations based on CD150 and CD48 expression, LSKCD150⁺CD48⁻ (A), LSKCD150⁺CD48⁺ (B), and LSKCD150⁻CD48⁺ (C). The LSKCD150⁺CD48⁻ (A) population can be further subdivided into CD135⁻CD34⁻ (HSC) and CD135⁻CD34⁺ (MPP1) subsets. Sets of representative FACS blots of Cdk6^+/+ and Cdk6^−/− BM cells are shown. (B) Total cell numbers of LSKCD150⁺CD48⁻ (A), LSKCD150⁺CD48⁺ (B), CD150⁻CD48⁺ (C) in Cdk6^+/+ and Cdk6^−/− animals are shown (n = 3 per genotype). (C) Analysis of individual subpopulations of LSKCD150⁺CD48⁻ (A) cells is depicted. Population A is further subdivided into HSC and MPP1 (n = 3 per genotype; *P < .05). (D) Cell-cycle distributions of fraction A cells were analyzed with DAPI and Ki-67 staining. One representative FACS blot per genotype is depicted (left). Summary of data (right; n = 6 per genotype).

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