Properties of NK cells expressing mbIL15. (A) Relative proportion of GFP⁺ cells before and after 7 days of culture among NK cell populations transduced with mbIL15 or mock transduced. Results with NK cells from 13 donors are shown (P < .001 for mbIL15, not significant for mock). (B) Immunophenotypic features of mbIL15-transduced NK cells. Cell marker analysis by flow cytometry was performed on NK cells cultured for 48 hours without IL-2. All results are summarized in supplemental Table 1. (C) Mock- and mbIL15-transduced NK cells were cultured for 48 hours without IL-2, and cell lysates were analyzed by Kinex Antibody Microarray (Kinexus, Vancouver, CA). Of 854 antiphosphoprotein antibodies tested, shown are those whose signals had a Z-ratio >0.5 and an error range <100%. Bars indicate percent signal change in NK cells expressing mbIL15 compared with the normalized intensity in mock-transduced NK cells.