The NF-κB p50 subunit differentially binds the Cxcr4 promoter in T cells of AA mice. (A) Schematic representation of the Cxcr4 promoter showing relative location of predicted binding sites for NF-κB subunits, p50 and c-Rel, and regions amplified by primer sets #1 and #2 (not shown to scale). (B) Representative image of agarose gel showing 2 amplified regions of the Cxcr4 promoter. Chromatin was from BM-infiltrating CD8⁺ cells of mice induced with AA (BMF; right) or spleen-infiltrating CD8⁺ T cells of noninduced mice (Naïve; left) immunoprecipitated using antibodies specific for p50 and c-Rel. (C) Quantification of band intensities of Naïve and BMF samples subjected to ChIP in (B). Data are representative of 2 independent experiments.