Reversal of inhibitor formation and anaphylaxis in hemophilia B mice. (A) Feeding and FIX administration schedule. Numbers in circle indicate time point of blood collection. (B) Inhibitor titers in BU/mL, (C) IgG1 titers against FIX at weeks 6, 15, and 21 of the experiment. Control mice were fed with WT plant material (control, black squares, n = 5). A second group received FIX transgenic plant material during weeks 6 to 14 followed by WT plant material (fed 1, gray triangles, n = 6), and a third group received FIX transgenic plant material for weeks 6 through 20 (fed 2, black triangles, n = 4). Data are average ± SEM. (D) Survival analysis in the same set of experimental mice. (E) IgE formation against FIX in plasma of the same mice.