Gene expression profiling analysis reveals dysregulation of miR-29a targets in HSCs. (A) Numbers of differentially expressed genes in miR-29a/b-1 KO or WT HSCs (Lin⁻c-Kit⁺Sca-1⁺CD34⁻Slam⁺) or committed progenitor (Lin⁻c-Kit⁺Sca-1⁻; Prog) cells. Genes were selected using a P value threshold of <.05 and a fold change >1.5. (B) Unsupervised clustering analysis of dysregulated genes identified in A. (C) Unsupervised clustering analysis of genes predicted to be targets of miR-29a. Predicted targets were identified using targetscan.org (Release 6.1). (D) Validation of changes in the expression levels of reported regulators of bone marrow HSPCs using c-Kit-enriched bone marrow cells from miR-29a/b-1 WT or KO mice by qRT-PCR. Fold change was calculated by comparing KO with WT cells. The genes shown represent predicted miR-29a target genes with fold change >1 in miR-29a/b-1 KOs compared with WT littermates. (E) Selected regulators of HSC function were chosen based on published genetic evidence. Fold change was relative to WT type. Error bars represent SEM from triplicate experiments.